Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Enzo Life Sciences Wash buffer concentrate, (100ml)
Formulation: Liquid. TRIS buffered saline containing detergents. Long Term Storage: Ambient. Applications: ELISA .
General Laboratory Products 2mL 0.85 per. Saline, 100/cs
2mL 0.85 per. Saline with Shelf Life of 1 yr.
Growcells PBS 1X 1L CS12
Ready to use, sterile 1X Phosphate-Buffered Saline solution prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 8g, KCl 0.2g, Na2HPO4 1.44g, KH2PO4 0.24g
Aniara Diagnostica LLC PBS PDR PH 7.4 10L
Medicago Phosphate Buffered Saline (PBS) pH 7.4 supplied as preweighed powder mix in sealed pouch yields 10 L 0.14 M NaCl, 0.0027 M KCl, 0.010 M phosphate buffer with pH 7.4 at 25C. Used as biological buffer, isotonic. 1 pouch.
Rockland Immunochemicals Normal Saline - MB-049-1000
This solution is commonly referred to as physiological saline or isotonic saline.
Apexbio Technology LLC Protease Inhibitor Cocktail (EDTA-Free 100X in DMSO) 100x1ml
Crude cell extracts contain a variety of endogenous enzymes capable of degrading proteins such as phosphatases and proteases The optimal method to enhance the yield of intact proteins is to supplement with inhibitors targeting these known enzymes This Protease Inhibitor Cocktail is used in plant cell and tissue extracts to improve protein stability The cocktail inhibits proteases that degrade both non-phosphorylated and phosphorylated protein substrates The Protease Inhibitor Cocktail contains individual components including AEBSF 1 10-Phenanthroline Bestatin E-64 Leupeptin and Pepstatin A providing broad specificity against cysteine serine aspartic metalloproteases and aminopeptidases This protease inhibitor cocktail has been optimized and tested specifically for plant cell and tissue extracts Provided as a ready-to-use solution this protease inhibitor cocktail is supplied in DMSO as the solvent
Apexbio Technology LLC Protease Inhibitor Cocktail for bacterial cell extracts (100 X in DMSO) 50x(1ml A 1ml B)
Under stable environmental conditions endogenous protein synthesis and degradation are balanced thus maintaining stable cellular protein levels Cell crude extracts contain numerous endogenous enzymes such as phosphatases and proteases that can degrade proteins in the extracts During in vitro protein extraction from cells and tissues protein synthesis practically ceases while protein degradation increases The optimal way to enhance yields of intact proteins is to add inhibitors for these known enzymes The Protease Inhibitor Cocktail for bacterial cell extracts (100X in DMSO) is designed for bacterial extracts to enhance the stability of proteins This cocktail inhibits proteases responsible for degrading non-phosphorylated or phosphorylated protein substrates This Protease Inhibitor Cocktail is provided as a ready-to-use solution and it is formulated in DMSO
ILEX LIFE SCIENCES LLC GELATIN SOLUTION 2.0 6 X 100ML
PAN-Biotech Gelatin Solution 2% in DPBS, w/o: Ca, Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech Gelatin Solution 2% in DPBS, w/o: Ca, Mg for laboratory research applications. Gelatin is a heterogenous mixture of water soluble proteins of high average molecular weight present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, or bones in water. APPLICATION: Applications using gelatin include coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to stabilize Taq DNA Polymerase, and use as a blocking agent in Western Blotting, ELISA, and immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media. STORAGE: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
A measured mixture of chemically defined ingredients makes up a basal medium which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/f-12 is a completely defined synthetic medium which is protein-free.
Apexbio Technology LLC Annexin V-APC/PI Apoptosis Kit 20tests
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (APC) can be used as a probe in combination with flow cytometry or fluorescence microscopy for detecting apoptosis and the staining process requires only 10-20 minutes Additionally this kit can be paired with the nuclear dye propidium iodide (PI) PI specifically binds to double-stranded DNA emitting red fluorescence upon binding but it cannot normally permeate intact cell membranes However PI can penetrate compromised cellular membranes in late-stage apoptotic and dead cells staining nuclei Therefore combined staining with Annexin V-APC and PI allows
Alkali Scientific CellPro™ PBS 10X, pH 7.2, without calcium, magnesium, 500mL, 500ml, 10/case
CellPro™ PBS, 10X, pH7.2, 500mL Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.
![Alkali Scientific PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 98.6g make, 1 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661308.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 98.6g make, 1 L
PBS [Phosphate Buffered Saline] 10X Powder Concentrate is a white granular powder that, when dissolved in water, creates a 10X concentrated PBS solution. This 98.6g pack makes 1 liter of 10X PBS, providing a convenient and efficient way to prepare large volumes of phosphate-buffered saline. The powder dissolves quickly and is ideal for laboratories that need a concentrated PBS solution for dilutions or experiments that require higher concentration buffers. PBS is widely used in cell culture, molecular biology, and protein studies, and the 10X concentrate is ideal for reducing storage space and simplifying buffer preparation in the lab.
Boston Bioproducts Inc Dulbecco's Phosphate Buffered Saline, DPBS - 20L-CBY
Dulbecco s Phosphate Buffered Saline DPBS - 20L-CBY (For Research Use and Further Manufacturing Only)